Inheritance of Normal Pod Development in Bean Golden Yellow Mosaic Resistant Common Bean

RNAi-mediated resistance to Bean golden mosaic virus in genetically engineered common bean (Phaseolus vulgaris).

Bean golden mosaic virus (BGMV) is transmitted by the whitefly Bemisia tabaci in a persistent, circulative manner, causing the golden mosaic of common bean (Phaseolus vulgaris L.). The characteristic symptoms are yellow-green mosaic of leaves, stunted growth, or distorted pods. The disease is the largest constraint to bean production in Latin America and causes severe yield losses (40 to 100%)....

GM plants with RNAi - golden mosaic resistant bean

RNA silencing is a biochemical mechanism that regulates gene expression by post-transcriptionally activating a sequence-specific RNA degradation by three different pathways: (i) small interfering RNA (siRNA) silencing of exogenous mRNA; (ii) micro RNA (miRNA) silencing of endogenous mRNAs, and (iii) associated with DNA methylation and suppression of transcription [2]. These processes share thre...

Purification and Serology of Bean Yellow Mosaic Virus

density-gradient centrifugation. ISCO fractionator scanning patterns indicated a high degree of purity but serological results revealed the presence of some host contaminates. The yield of virus was abeut 2mgfleOg of bro'ad bean leaves. The titer of the antiserum against isolate No. 30 was 1!2048 by ring precipitin tests. Although the iutact virus particles could n6t diffuse in agar gel plates,...

Determination of Carotene Content in Yellow-seeded Common Bean Varieties

Introduction Yellow-seeded beans are a minor commercial grain type from the Andean gene pool that are eaten in a subset of Latin American bean-producing countries, notably in Peru, Mexico and Brazil. Recently, they have become embroiled in a controversy in the United States where a patent was claimed on the marketing of yellow beans. In the meantime, the scientific basis for the yellow color is...

Sequence Analysis of Expressed cDNA of Bean Common Mosaic Virus RU1 Isolate